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KMID : 0900919950190020153
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Korean journal of Animal Reproduction
1995 Volume.19 No. 2 p.153 ~ p.157
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Studies on the Microbe Control at Bovine Embryo Production by In Vitro Fertilization
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Lee Myung -Shik
Ko Yeoung-Gyu
Yang B.-S.
Oh S.-J.
Park Y.-Y.
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Abstract
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These studies were conducted to investigate the microbe control effect of antibiotics treatment in all media used for in vitro fertilization(IVF) embryo production. The bovine oocytes were recovered from follicles(2~5mm) and were cultrued for 22hrs at 38.5^{circ}C with 5% CO2 incubator. The contamination and development rate in vitro fertilized oocyte was evaluated everyday. The results were summerized as follow ; 1. Control, antibiotic-antimycotic solution(AAS, Gibco) 1%＋gentamycin, and AAS 1%＋kanamycin(Sigma, USA) treatment was contaminated with 72hrs. However Baytril and Kanamycin(Korea) added was not contaminated. 2. The blastocyst formation rate in Baytril supplementated 1, 2 and 3{mu}ell/ml was 3.73, 1.28 and 0.00%, respectively. 3. The blastocyst formation in kanamycin added concentration of 50, 75 and 100mutextrm{g}/ml was 13.0, 9.4 and 3.49%, respectively.
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KEYWORD
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bovine in vitro fertilization, contamination, blastocyst
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